Structural and dynamic characterisation of biomolecular protein complexes is the key for understanding essential biological processes and mechanisms and their role in diseases. The complexity of these systems, as well as the necessity to measure them in their native state, i.e. in solution, renders them difficult for conventional structural biology techniques.
The combination of small-angle X-ray scattering (SAXS) and nuclear magnetic resonance (NMR) spectroscopy meets this requirement: both methods complement each other in a way that SAXS reveals the overall three-dimensional shape and size of biomolecular complexes in solution and NMR provides high-resolution information on inter-atomic distances, binding interfaces and orientation of subunits. Both SAXS data and NMR data are combined in a modelling step for determining the complex structure.
Please download the application note for further information.
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