Small-angle X-ray scattering is a widely used technique in structural biology for the determination of protein size and shape. Biological macromolecules in solution can exist in numerous conformations and generally have a strong tendency for agglomeration, making data evaluation of such molecules without prior purifications sometimes tedious. Size-exclusion chromatography (SEC) is capable of separating molecules by size. In-line SEC in combination with SAXS can considerably improve data quality and thus the accuracy of the data evaluation.
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