Ventilator-on-a-chip compares injury caused by mechanical ventilation

In a new study, using a ventilator-on-a-chip model developed at The Ohio State University, researchers found that shear stress from the collapse and reopening of the air sacs is the most harmful type of damage.

This miniature organ-on-a-chip model simulates lung injury during mechanical ventilation plus repair and recovery in human-derived cells in real time, said co-lead author Samir Ghadiali, PhD, professor and chair of biomedical engineering at Ohio State.

“The initial damage is purely physical, but the processes after that are biological in nature – and what we’re doing with this device is coupling the two,” Ghadiali said in a statement.

The team hopes the device will also help in the development of therapies to address ventilator-induced lung injury.

“This is an important advance in the field that will hopefully allow for a better understanding of how lung injury develops in mechanically ventilated patients and identification of therapeutic targets so that we can give drugs to prevent that kind of injury or treat it when it happens,” said co-lead author Joshua Englert, MD, associate professor of pulmonary, critical care and sleep medicine at The Ohio State University Wexner Medical Center.

The research is published in Lab on a Chip.

It has long been known that the mechanical forces exerted on the lungs by ventilators can cause injury. The damage at the cellular level can make the barrier between air sacs and capillaries carrying blood become leaky, leading to fluid build-up that interferes with oxygen getting to the lungs.

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According to the University, particular value is the ventilator-on-a chip’s measurement of real-time changes to cells that affect the integrity of that barrier, enabled by an innovative approach: growing human lung cells on a synthetic nanofibre membrane mimicking the complex lung matrix. It’s closer to the authentic ventilated lung microenvironment than any similar lung chip systems to date, the researchers said.

The device measures the effects of three types of mechanical stress on the integrity of the barrier: lung cell stretch from over inflation, increased pressure on lung cells, and cyclical collapse and reopening of air sacs.

Experiments showed that over inflation with a high volume of air and cyclic collapse and reopening of air sacs both led the barrier to become leaky, but the cells could recover more quickly from over inflation than from the repetitive opening and closing of air sacs.

Englert said the collapse and reopening may be more problematic because it makes fluid in the lungs move, exposing cells to high amounts of shear stress.

“There really hasn’t been a lot of data that could allow for the comparison of those two injurious forces in the same system,” he said. “But now for the first time, we can use the same device with the same cells and induce both types of injury and see what happens. Our data suggests neither one of them is good, they’re both injurious, but that the collapse and reopening seems to be more severe and makes recovery harder.”

This finding was a demonstration of the model’s sophistication, Ghadiali said.

“We knew for a long time that collapse and reopening is a pretty injurious force, but we never could measure it in real time,” he said. “Now that we know that collapse and reopening injury happens much quicker and takes a long time to recover, we plan to use the ventilator on a chip to figure out how to prevent this injury and/or enhance the repair.”

Next steps involve modelling diseases such as pneumonia and traumatic injuries experienced by ICU patients in combination with mechanical action.