Vein-on-a-chip could improve thrombosis research

The vein-on-a-chip model has been developed by scientists at Birmingham University and could be used in experiments to understand mechanisms of blood clot formation.

Deep vein thrombosis is the development of blood clots in veins, usually in the legs. It is a serious condition because the clot can detach and travel to the lungs, where it may block blood vessels, causing difficulty in breathing that may be fatal. Deep vein thrombosis is a third most common cardiovascular disease after myocardial infarction and stroke, with tens of thousands of people in the UK developing this condition every year.

Mechanisms of deep vein thrombosis require further research to improve clinicians’ understanding and ability to treat or prevent the condition.

Described in a recent paper published in Frontiers in Cardiovascular Medicine, the Birmingham team’s device is a tiny channel, which includes structures called ‘valves’ that ensure the correct direction of blood flow.

Dr Alexander Brill from Birmingham’s Institute of Cardiovascular Sciences, together with Drs Daniele Vigolo and Alessio Alexiadis from the School of Chemical Engineering, led the development of the new device.

Dr Brill said that the device is more advanced than previous models because the valves can open and close, mimicking the mechanism seen in a real vein.

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“It also contains a single layer of cells, called endothelial cells, covering the inside of the vessel,” he said. “These two advances make this vein-on-a-chip a realistic alternative to using animal models in research that focuses on how blood clots form. It is biologically reflective of a real vein, and it also recapitulates blood flow in a life-like manner.”

According to the team, they were able to demonstrate one of the basic mechanisms underlying venous clot formation using their newly developed model. Namely, the role of a bridge between a molecule called von Willebrand Factor and a surface receptor on platelets called glycoprotein Ib-alpha.

“Organ-on-a-chip devices, such as ours, are not only created to help researchers move away from the need for animal models, but they also advance our understanding of biology as they are more closely representative of how the human body works,” Dr Brill added.

“The principles of the 3Rs – to replace, reduce and refine the use of animals in research – are embedded in national and international legislation and regulations on the use of animals in scientific procedures. But there is always more that can be done. Innovations such as the new device created for use in thrombosis research are a step in the right direction.”