Researchers look at human tissues with microscopy

According to NCSU, the study focused specifically on eye tissues, which are damaged by scarring in diabetic patients.

‘Our findings are a proof of concept, showing that this technique is extremely effective at giving us the data we need on these tissues,’ said Dr Albena Ivanisevic, co-author of a paper describing the research. ‘Specifically, it gives a great deal of information on the composition of these tissues, as well as the tissue’s topography, or surface characteristics.’

According to a statement, the study is one of the first to explore how this technology, called bimodal dual AC mode microscopy, can improve understanding of human tissues and biomaterials.

The research team, which included researchers from Purdue University and the University of Louisville School of Medicine, examined two types of eye tissue from diabetic patients.

Specifically, they looked at the inner limiting membrane (ILM), which is the surface layer of the retina, and so-called epiretinal membranes. Epiretinal membranes are scar tissues that form on the ILM in diabetics. Scar tissue can cause significant damage to the retina and, if untreated, may lead to blindness.

There are multiple treatments for this scarring. In the US, a common technique is for a surgeon to peel off the ILM, removing the scar tissue with it. In many other parts of the world, surgeons inject dye into the eye to better distinguish the parts of the eye they will operate on. This process is not currently allowed in the US, due to concerns about the dye’s toxicity.

The researchers launched this project, in part, to determine if bimodal dual AC mode microscopy could be used to provide a better understanding of the topographical properties of the ILM.

Further, the researchers wanted to use the technology to see if it offered insight into how — or whether — various dyes affect the topographical characteristics of the ILM.

The researchers found that bimodal dual AC mode microscopy, an atomic force imaging technique, captured the properties of the tissue in exceptional detail. Atomic force imaging effectively runs a probe over the surface of a material to collect data on its topography, similar to the way in which a record player’s needle runs over the surface of an album.

‘The next step would be to use this technology to assess the utility — and potential risk — of various dyes,’ Ivanisevic said. ‘If we can find a dye that is extremely effective and poses little risk, it may be approved for use in future surgeries.’

The paper, ‘Deposition of Triamcinolone Acetonide and Its Effect on Soft Tissue Topography’, was published online on 5 June in Advanced Healthcare Materials.